Coding

Part:BBa_K576002:Design

Designed by: George Zarubin   Group: iGEM11_Waterloo   (2011-06-20)


GFP2 TailPart


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 186
    Illegal SapI site found at 3


Design Notes

The reading frame fix in the front is unnecessary. The part is in recommended RFC53 D-Tail format, i.e. the overhang is 5' ATG.


Source

BBa_I715020

References